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The data obtained after 30 h incubation of yeast in media supplemented with 40 and 60 mg Se 4+ /L culture media showed a slight increase in the OD as compared to the culture obtained after 24 h incubation

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Seawater was used for a negative control to observe animal response to the input flow and 1 mL of feeding solution made using 2 Tetra Tabimin dissolved in 200 mL of water as a positive control